Fluorescence Filter Set, ODiate®, DAPI Fluorophore

The OD8-FF2005 ODiate^® Fluorescence Filter Set has been designed for excellent epi-fluorescence imaging of DAPI and similar fluorophores. The filter set includes a Excitation Filter, a Dichroic Beamsplitter, and a Emission Filter. ODiate Fluorescence Filters are manufactured with a dense sputtering process to meet the highest demands for fluorescence and microscopy applications including exceptionally high passband transmission, maximized out-of-band blocking ≥ OD8, extremely steep slopes, and spectral specifications illuminating fluorescence chemistries to provide maximum image brightness.

Alternate Fluorophores

In addition to DAPI the following fluorophores are also compatible with this set:

Alexa Fluor^® 350, AMCA, ATTO 390, CF350, EBFP, Brilliant Violet 421™, HCS CellMask Blue, Hoechst^® 33258, Marina Blue^®, Pacific Blue, PromoFluor-350, PromoFluor-370LSS, sgBFP, Spectrum Blue, TagBFP, Vybrant^® DyeCycle Violet.

Optimized Coatings and Engineering Expertise

Newport pushed the boundaries with the introduction of our ODiate Fluorescence Filter Sets, to allow customers to fully optimize their system performance. Our expert engineering team has been involved from the first stage of design to ensure that we exceed your requirements and continues through the entire process of continual production deliveries.

Environmental Stability and Physical Durability

The coating systems used to produce ODiate filters use a dense sputtering process to provide robust environmental reliability to meet the highest demands for thin-film coatings. The resulting highly durable coatings meet or exceed testing procedures including humidity, thermal shock, adhesion, chemical resistance, and moderate abrasion per U.S. Military and international standards.

Specifications

Technical Specs

Transmission
Excitation & Emission Filters: Tavg ≥ 96% over passband
Reflection
Dichroic Beamsplitters: Ravg ≥ 98% over Excitation passband
Angle of Incidence
Excitation & Emission Filters: 0-5°
Dichroic Beamsplitters: 45±1.5°
Parallelism
Excitation & Emission Filters: ≤ 10 arcseconds
Dichroic Beamsplitters: ≤ 5 arcsecond
Material
Excitation & Emission Filters: BOROFLOAT® 33
Dichroic Beamsplitters: Fused Silica

Dimensions
Excitation & Emission Filters: 25 mm
Dichroic Beamsplitters: 25.2 x 35.6 mm
Fluorophores
DAPI
Surface Quality
60-40 Scratch-Dig over clear aperture

Transmission
Excitation & Emission Filters: Tavg ≥ 96% over passband
Reflection
Dichroic Beamsplitters: Ravg ≥ 98% over Excitation passband
Angle of Incidence
Excitation & Emission Filters: 0-5°
Dichroic Beamsplitters: 45±1.5°
Parallelism
Excitation & Emission Filters: ≤ 10 arcseconds
Dichroic Beamsplitters: ≤ 5 arcsecond
Material
Excitation & Emission Filters: BOROFLOAT® 33
Dichroic Beamsplitters: Fused Silica

Dimensions
Excitation & Emission Filters: 25 mm
Dichroic Beamsplitters: 25.2 x 35.6 mm
Fluorophores
DAPI
Surface Quality
60-40 Scratch-Dig over clear aperture

Resources & Downloads

Literature

ODiate Fluorescence Filter Sets Datasheet(1.7 MB, PDF)