Fluorescence Up conversion

In fluorescent up conversion, the fluorescent sample is pumped by a femtosecond pulse and the fluorescence is up converted using an optically gated pulse to generate sum frequency radiation. The up converted light is then spectrally resolved via monochromator or spectrograph using a PMT. The software automatically controls the up conversion crystal’s phase matching angle and monochromator grating angle throughout the fluorescent wavelength range of interest. The software also controls the delay line and gathers time-resolved information which is displayed in a 3D color plot. Calibrated GVD and photometric corrections of spectra are also automated in the software. Compared to pump-probe spectroscopy techniques such as transient absorption, the data interpretation of fluorescence up conversion is straightforward as only emission is measured. Fluorescence up conversion offers fs-resolved dynamics information over a range of 3.3 ns in the UV-VIS and IR spectral ranges.

Fluorescence upconversion dynamics of Coumarin 153 in MeOH.

Time-correlated Single Photon Counting Option

For researchers interested in studying time dynamics out to µs time range, time-correlated single photon counting can be added primarily as an option for systems being pumped by femto or picosecond oscillators. TCSPC covers the UV-VIS wavelength ranges with high resolution. The TCSPC method is highly sensitive, so this will work well even for weak signals.

Comparison of Fluorescence Upconversion to TCSPC for C153 in MeOH. Fluorescence upconversion captures early dynamics while TCSPC captures extended time scale dynamics.


Applications include:

  • Ultrafast dynamics of chemical reactions
  • Excited state dynamics
  • Conformation dynamics and kinetics in fluorescent molecules
    Quantum dots characterization
  • Bioorganic photosynthetic system analysis
  • Dynamics in fluorescent proteins and DNA constituents
  • Carrier dynamics in nanocrystals and nanoparticles
  • Proton transfer, and dye/label photophysics