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Home > Products > Optical Filters > Fluorescence Filters > High Performance Filters for Fluorescence Microscopy

High Performance Filters for Fluorescence Microscopy
High Performance Filters for Fluorescence Microscopy
  • Patented Stabilife® Coatings
  • Shift free spectral response @ 0% - 100%RH
  • Humidity per MIL-STD-810, Method 507.3, Procedure III, 20 cycles
  • Lowest autofluorescence
  • Extremely sharp transitions from rejection to transmission regions
  • Maximum transmission
  • Excellent set signal-to-noise ratio
  • Optimized optical fabrication for brilliant imaging
  • Superior field life

Product DescriptionProduct DetailSpecificationsDownloads
Specifications

HPF™ Fluorescence Filters - Typical Specifications
   Excitation Filters  Dichroic Filters  Emission Filters
 Passband Transmittance  ≥90%  ≥90%  ≥90%
 Dichroic Reflectance  N/A  ≥90%  N/A
 Spectral Blocking  ≥OD6  N/A  ≥OD6
 Surface Quality  E/E (60/40) per MIL-F-48616 (typical)  E/E (60/40) per MIL-F-48616 (typical)  E/E (60/40) per MIL-F-48616 (typical)
 Coating Hardness  MIL-C-48497  MIL-C-48497  MIL-C-48497
 Coating Abrasion Resistance  MIL-C-48497  MIL-C-48497  MIL-C-48497
 Coating Adhesion  MIL-C-48497  MIL-C-48497  MIL-C-48497
 Coating Humidity Resistance  MIL-STD-810, Method 507.3, Procedure III, Modified to 40 cycles**  MIL-STD-810, Method 507.3, Procedure III, Modified to 40 cycles**  MIL-STD-810, Method 507.3, Procedure III, Modified to 40 cycles**
 Coating Operating Temperature Range  -100 º C to 300 º C**  -100 º C to 300 º C**  -100 º C to 300 º C**
 Filter Size  25 mm dia.  25.5 mm x 36 mm  25 mm dia.
 Filter Thickness  5 mm  1 mm  5 mm

**The specifications for humidity resistance and coating operating temperature range listed above apply to exposed coatings only. Humidity resistance and operating temperature range of filters manufactured using Stabilife® coatings and assembled using epoxy systems revert to the humidity resistance and operating temperature range of the epoxy system.

HPF™ Fluorescence Filter Sets - Fluorophore Reference
 Fluorophore  Filter Set  Excitation Filter  Dichroic Filter  Emission Filter
 5-FAM  HPF1245  HPX475-44  HPD505  HPM535-50
 5-ROX  HPF1340  HPX560-50  HPD590  HPM640-60
 5-TAMRA  HPF1335  HPX525-45  HPD560  HPM620-75
 6-HEX  HPF1295  HPX520-40  HPD550  HPM590-55
 6-JOE  HPF1230  HPX500-20  HPD515  HPM530LP
 6-TET  HPF1280  HPX500-40  HPD530  HPM575-50
 Alexa Fluor® 350  HPF1205  HPX360-60  HPD400  HPM460-60
 Alexa Fluor® 430  HPF1255  HPX425-40  HPD490  HPM540-40
 Alexa Fluor® 488  HPF1245  HPX475-44  HPD505  HPM535-50
 Alexa Fluor® 532  HPF1275  HPX500-40  HPD530  HPM560-50
 Alexa Fluor® 546  HPF1300  HPX525-45  HPD560  HPM600-60
 Alexa Fluor® 555  HPF1300  HPX525-45  HPD560  HPM600-60
 Alexa Fluor® 568  HPF1345  HPX560-40  HPD595  HPM640-60
 Alexa Fluor® 594  HPF1345  HPX560-40  HPD595  HPM640-60
 Alexa Fluor® 633  HPF1385  HPX610-60  HPD645  HPM685-50
 Alexa Fluor® 647  HPF1400  HPX620-50  HPD660  HPM700-60
 Alexa Fluor® 660  HPF1410  HPX630-60  HPD680  HPM720-60
 Alexa Fluor® 680  HPF1415  HPX660-40  HPD695  HPM725-40
 Alexa Fluor® 700  HPF1420  HPX660-40  HPD695  HPM740-50
 Alexa Fluor® 750  HPF1430  HPX710-50  HPD760  HPM810-80
 AMCA  HPF1205  HPX360-60  HPD400  HPM460-60
 BODIPY® 630/650  HPF1380  HPX605-40  HPD635  HPM670-50
 BODIPY® 650/665  HPF1395  HPX630-40  HPD660  HPM695-50
 BODIPY® FL  HPF1250  HPX480-40  HPD505  HPM535-50
 BODIPY® R6G  HPF1285  HPX510-40  HPD540  HPM575-50
 BODIPY® TMR  HPF1305  HPX530-40  HPD560  HPM600-60
 BODIPY® TR  HPF1355  HPX570-40  HPD610  HPM645-50
 Calcium Crimson™  HPF1365  HPX570-40  HPD595  HPM645-60
 CFP  HPF1220  HPX440-20  HPD455  HPM480-40
 Cy3.5™  HPF1325  HPX565-30  HPD580  HPM620-60
 Cy3™  HPF1310  HPX535-40  HPD565  HPM610-50
 Cy5.5™  HPF1405  HPX645-40  HPD675  HPM710-50
 Cy5™  HPF1390  HPX620-50  HPD655  HPM690-50
 Cy7™  HPF1425  HPX710-50  HPD755  HPM800-70
 DAPI  HPF1205  HPX360-60  HPD400  HPM460-60
 Dil(rs)  HPF1315  HPX535-50  HPD565  HPM620-60
 DiO  HPF1250  HPX480-40  HPD505  HPM535-50
 DsRed  HPF1320  HPX545-30  HPD570  HPM620-60
 Eosin  HPF1270  HPX500-40  HPD525  HPM560-50
 Ethidium Bromide  HPF1350  HPX525-45  HPD565  HPM640-70
 FITC  HPF1250  HPX480-40  HPD505  HPM535-50
 Fluo 3  HPF1250  HPX480-40  HPD505  HPM535-50
 Fluo 4  HPF1250  HPX480-40  HPD505  HPM535-50
 GFP  HPF1240  HPX470-40  HPD495  HPM535-50
 GFP(rs)  HPF1250  HPX480-40  HPD505  HPM535-50
 GFP(wt)  HPF1225  HPX450-50  HPD480  HPM510-50
 Hoechst 33342 & 33258  HPF1200  HPX350-60  HPD400  HPM460-60
 LysoSensor™ Blue (pH 5)  HPF1210  HPX380-40  HPD410  HPM460-60
 MitoTracker® Orange  HPF1335  HPX525-45  HPD560  HPM620-75
 MitoTracker® Red  HPF1340  HPX560-50  HPD590  HPM640-60
 Nile Red  HPF1360  HPX525-45  HPD565  HPM645-60
 Oregon Green™  HPF1260  HPX485-30  HPD515  HPM545-30
 Pacific Blue™  HPF1215  HPX400-30  HPD440  HPM470-30
 PE (B,R)  HPF1290  HPX525-45  HPD560  HPM585-40
 Propidium Iodide  HPF1370  HPX525-45  HPD565  HPM645-75
 Resorufin  HPF1375  HPX560-50  HPD595  HPM645-75
 Rhodamine  HPF1330  HPX570-20  HPD585  HPM620-60
 Rhodamine Green™  HPF1235  HPX500-20  HPD515  HPM535-30
 Texas Red®  HPF1375  HPX560-50  HPD595  HPM645-75
 TRITC  HPF1300  HPX525-45  HPD560  HPM600-60
 YFP  HPF1265  HPX500-20  HPD525  HPM550-40
 YGFP  HPF1235  HPX500-20  HPD515  HPM535-30


HPF™ Fluorescence Filter Sets - Numerical Reference
 Model  Fluorophore  Excitation  Dichroic  Emission
 HPF1200  Hoechst 33342 & 33258  HPX350-60  HPD400  HPM460-60
 HPF1205  Alexa Fluor® 350, AMCA, DAPI  HPX360-60  HPD400  HPM460-60
 HPF1210  LysoSensor™ Blue (pH5)  HPX380-40  HPD410  HPM460-60
 HPF1215  Pacific Blue™  HPX400-30  HPD440  HPM470-30
 HPF1220  CFP  HPX440-20  HPD455  HPM480-40
 HPF1225  GFP (wt)  HPX450-50  HPD480  HPM510-50
 HPF1230  6-JOE  HPX500-20  HPD515  HPM530-LP
 HPF1235  Rhodamine Green™, YGFP  HPX500-20  HPD515  HPM535-30
 HPF1240  GFP  HPX470-40  HPD495  HPM535-50
 HPF1245  5-FAM, Alexa Fluor® 488  HPX475-44  HPD505  HPM535-50
 HPF1250  BODIPY® FL, DiO, FITC, Fluo 3, Fluo 4, GFP (rs)  HPX480-40  HPD505  HPM535-50
 HPF1255  Alexa Fluor® 430  HPX425-40  HPD490  HPM540-40
 HPF1260  Oregon Green™  HPX485-30  HPD515  HPM545-30
 HPF1265  YFP  HPX500-20  HPD525  HPM550-40
 HPF1270  Eosin  HPX500-40  HPD525  HPM560-50
 HPF1275  Alexa Fluor® 532  HPX500-40  HPD530  HPM560-50
 HPF1280  6-TET  HPX500-40  HPD530  HPM575-50
 HPF1285  BODIPY® R6G  HPX510-40  HPD540  HPM575-50
 HPF1290  PE (B,R)  HPX525-45  HPD560  HPM585-40
 HPF1295  6-HEX  HPX520-40  HPD550  HPM590-55
 HPF1300  Alexa Fluor® 546, Alexa Fluor® 555, TRITC  HPX525-45  HPD560  HPM600-60
 HPF1305  BODIPY® TMR  HPX530-40  HPD560  HPM600-60
 HPF1310  Cy3™  HPX535-40  HPD565  HPM610-50
 HPF1315  Dil (rs)  HPX535-50  HPD565  HPM620-60
 HPF1320  DsRed  HPX545-30  HPD570  HPM620-60
 HPF1325  Cy3.5™  HPX565-30  HPD580  HPM620-60
 HPF1330  Rhodamine  HPX570-20  HPD585  HPM620-60
 HPF1335  5-TAMRA, MitoTracker® Orange  HPX525-45  HPD560  HPM620-75
 HPF1340  5-ROX, MitoTracker® Red  HPX560-50  HPD590  HPM640-60
 HPF1345  Alexa Fluor® 568, Alexa Fluor® 594  HPX560-40  HPD595  HPM640-60
 HPF1350  Ethidium Bromide  HPX525-45  HPD565  HPM640-70
 HPF1355  BODIPY® TR  HPX570-40  HPD610  HPM645-50
 HPF1360  Nile Red  HPX525-45  HPD565  HPM645-60
 HPF1365  Calcium Crimson™  HPX570-40  HPD595  HPM645-60
 HPF1370  Propidium Iodide  HPX525-45  HPD565  HPM645-75
 HPF1375  Resorufin, Texas Red®  HPX560-50  HPD595  HPM645-75
 HPF1380  BODIPY® 630/650  HPX605-40  HPD635  HPM670-50
 HPF1385  Alexa Fluor® 633  HPX610-60  HPD645  HPM685-50
 HPF1390  Cy5™  HPX620-50  HPD655  HPM690-50
 HPF1395  BODIPY® 650/665  HPX630-40  HPD660  HPM695-50
 HPF1400  Alexa Fluor® 647  HPX620-50  HPD660  HPM700-60
 HPF1405  Cy5.5™  HPX645-40  HPD675  HPM710-50
 HPF1410  Alexa Fluor® 660  HPX630-60  HPD680  HPM720-60
 HPF1415  Alexa Fluor® 680  HPX660-40  HPD695  HPM725-40
 HPF1420  Alexa Fluor® 700  HPX660-40  HPD695  HPM740-50
 HPF1425  Cy7™  HPX710-50  HPD755  HPM800-70
 HPF1430  Alexa Fluor® 750  HPX710-50  HPD760  HPM810-80


HPF™ Fluorescence Filter Set - Spectral Data

Fluorophores: Hoechst 33342, Hoechst 33258

Fluorophores: Alexa Fluor® 350, AMCA, DAPI

Fluorophores: LysoSensor™ Blue (ph5)

Fluorophores: Pacific Blue™

Fluorophores: CFP

Fluorophores: GFP (wt)

Fluorophores: 6-JOE

Fluorophores: Rhodamine Green™, YGFP

Fluorophores: GFP

Fluorophores: 5-FAM, Alexa Fluor® 488

Fluorophores: BODIPY® FL, DiO, FITC, Fluo 3, Fluo 4, GFP (rs)

Fluorophores: Alexa Fluor® 430

Fluorophores: Oregon Green™

Fluorophores: YFP

Fluorophores: Eosin

Fluorophores: Alexa Fluor® 532

Fluorophores: 6-TET

Fluorophores: BODIPY® R6G

Fluorophores: PE (B,R)

Fluorophores: 6-HEX

Fluorophores: Alexa Fluor® 546, Alexa Fluor® 555, TRITC

Fluorophores: BODIPY® TMR

Fluorophores: Cy3™

Fluorophores: Dil (rs)

Fluorophores: DsRed

Fluorophores: Cy3.5™

Fluorophores: Rhodamine

Fluorophores: 5-TAMRA, MitoTracker® Orange

Fluorophores: 5-ROX, MitoTracker® Red

Fluorophores: Alexa Fluor® 568, Alexa Fluor® 594

Fluorophores: Ethidium Bromide

Fluorophores: BODIPY® TR

Fluorophores: Nile Red

Fluorophores: Calcium Crimson™

Fluorophores: Propidium Iodide

Fluorophores: Resorufin, Texas Red®

Fluorophores: BODIPY® 630/650

Fluorophores: Alexa Fluor® 633

Fluorophores: Cy5™

Fluorophores: BODIPY® 650/665

Fluorophores: Alexa Fluor® 647

Fluorophores: Cy5.5™

Fluorophores: Alexa Fluor® 660

Fluorophores: Alexa Fluor® 680

Fluorophores: Alexa Fluor® 700

Fluorophores: Cy7™

Fluorophores: Alexa Fluor® 750
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