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Our High Signal-to-Noise Fluorescence Filters are designed for use in systems where high signal-to-noise ratios of each filter must be established independently. Many fluorescence detection filters, being developed for epi-fluorescence microscopy applications, depend upon pairing to achieve pair-specific signal-to-noise ratios. High S/N fluorescence filters do not have this limitation. Any filter High S/N filter can be paired with any other filter in the series, and deliver the same exceptional pair signal-to-noise ratio as would have been achieved with its traditional pair. The filters are designed with bandwidths consistent with the absorptance or emission spectra of the target fluorophore, but narrower to accommodate non-traditional pairing.
Using a multi-cavity film design and multiple blocking components, typical spectral rejection (blocking) of ≤1.0 x 10-8 is achieved from UV to 925nm. This provides integrated blocking of ≥OD5 when used with a tungsten source operating at 2800 °K and an S20 or equivalent photocathode detector. |
Specifications |
| Diameter |
25.4 mm+0/-0.5 mm |
| Minimum Active Area |
20.2 mm |
| Surface Quality |
80-50 scratch-dig; F/F per MIL-F-48616 |
| Thickness |
1.0 to 9.0 mm |
| Effective Index of Refraction ne |
1.5 |
| Wavelength Shift with Temperature |
0.01 nm/°C |
| Temperature Range |
-50°C to +100 °C |
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| Model |
Center Wavelength
(nm) |
FWHM
(nm) |
Minimum Peak Transmission
(%) |
Signal-to-Noise Ratio |
Integrated Blocking Range |
Corion Model |
| 10XM35-360 |
360, +0/-5 |
35±3.5 |
45 |
10,000:1 |
X-ray to 700 nm |
XM-360F |
| 10XM35-430 |
425, +5/-0 |
35±3.5 |
50 |
100,000:1 |
X-Ray to 925 nm |
XM-430F |
| 10XM35-465 |
460, +5/-0 |
35±3.5 |
50 |
100,000:1 |
X-Ray to 925 nm |
XM-465F |
| 10XM20-485 |
485, +0/-5 |
20±2.0 |
60 |
100,000:1 |
X-Ray to 925 nm |
XM-485F |
| 10XM25-530 |
530, +0/-5 |
25±2.5 |
60 |
100,000:1 |
X-Ray to 925 nm |
XM-530F |
| 10XM25-535 |
530, +5/-0 |
25±2.5 |
60 |
100,000:1 |
X-Ray to 925 nm |
XM-535F |
| 10XM25-540 |
540, +0/-5 |
25±2.5 |
60 |
100,000:1 |
X-Ray to 925 nm |
XM-540F |
| 10XM10-550 |
550, +0/-5 |
10±1.0 |
50 |
100,000:1 |
X-Ray to 1.2 µm |
XM-550F |
| 10XM10-585 |
580, +5/-0 |
10±1.0 |
50 |
100,000:1 |
X-Ray to 1.2 µm |
XM-585F |
| 10XM20-590 |
590, +0/-5 |
20±2.0 |
65 |
100,000:1 |
X-Ray to 925 nm |
XM-590F |
| 10XM35-595 |
590, +5/-0 |
35±3.5 |
65 |
100,000:1 |
X-Ray to 925 nm |
XM-595F |
| 10XM35-625 |
620, +5/-0 |
35±3.5 |
65 |
100,000:1 |
X-Ray to 925 nm |
XM-625F |
| 10XM35-635 |
630, +5/-0 |
35±3.5 |
65 |
100,000:1 |
X-Ray to 925 nm |
XM-635F |
| 10XM40-650 |
645, +5/-0 |
40±4.0 |
65 |
100,000:1 |
X-Ray to 925 nm |
XM-650F |
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Bandshape specifications and marker wavelengths are provided as approximate reference data only
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Integrated Cross-Talk Data for Fluorophore-Specific Filter Pairs |
| Dye |
Wavelength of Max Absorption |
Wavelength of Max Emission |
Excitation Filter |
Emission Filter |
Integrated Cross-Talk (Reference Only) |
| Aminomethylcoumarin (AMC) |
345 |
442 |
10XM35-360 |
10XM35-430 |
1,000,000:1 |
DAPI
Hoechst 33258
4-Methylumbeliferone
CPM |
358
351
360
385 |
458
455
450
471 |
10XM35-360 |
10XM35-465 |
100,000,000:1 |
| Lucifer Yellow |
430 |
540 |
10XM35-430 |
10XM25-535 |
1,000,000:1 |
| Acridine Orange (+RNA) |
460 |
650 |
10XM35-465 |
10XM35-625 |
1,000,000:1 |
YOYO-1 (+DNA)
Fluorescein (FITC)
Acridine Orange (+DNA)
BODIPY FL
Rhodamine 123 |
491
495
502
503
505 |
509
519
526
512
528 |
10XM20-485 |
10XM25-535 |
100,000,000:1 |
| Ethidium Bromide (+DNA) |
510 |
595 |
10XM20-485 |
10XM35-595 |
100,000,000:1 |
| Tetramethyrhodamine (TRITC) |
552 |
580 |
10XM25-530 |
10XM35-595 |
10,000,000:1 |
| R-phycoerythrin |
565 |
575 |
10XM20-485 |
10XM10-585 |
1,000,000:1 |
Ethidium Homodimer-1
Propidium Iodide |
528
536 |
617
617 |
10XM25-530 |
10XM35-625 |
100,000,000:1 |
| 7-Aminoactinomycin (7-AAD) |
550 |
645 |
10XM25-530 |
10XM40-650 |
1,000,000,000:1 |
| Texas Red |
595 |
615 |
10XM20-590 |
10XM35-635 |
100,000:1 |
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