Our High Signal-to-Noise Fluorescence Filters are designed for use in systems where high signal-to-noise ratios of each filter must be established independently. Many fluorescence detection filters, being developed for epi-fluorescence microscopy applications, depend upon pairing to achieve pair-specific signal-to-noise ratios. High S/N fluorescence filters do not have this limitation. Any filter High S/N filter can be paired with any other filter in the series, and deliver the same exceptional pair signal-to-noise ratio as would have been achieved with its traditional pair. The filters are designed with bandwidths consistent with the absorptance or emission spectra of the target fluorophore, but narrower to accommodate non-traditional pairing.Using a multi-cavity film design and multiple blocking components, typical spectral rejection (blocking) of ≤1.0 x 10-8 is achieved from UV to 925nm. This provides integrated blocking of ≥OD5 when used with a tungsten source operating at 2800 °K and an S20 or equivalent photocathode detector.