High Performance Filters for Fluorescence Microscopy
  • Patented Stabilife® Coatings
  • Shift free spectral response @ 0% - 100%RH
  • Humidity per MIL-STD-810, Method 507.3, Procedure III, 20 cycles
  • Lowest autofluorescence
  • Extremely sharp transitions from rejection to transmission regions
  • Maximum transmission
  • Excellent set signal-to-noise ratio
  • Optimized optical fabrication for brilliant imaging
  • Superior field life
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General Description Models

Newport HPF™ Filters for Microscopy Applications

Newport's HPF™ series of filters for fluorescence detection is a compilation of product designs of filters that was developed to be used with many of the most popular standard fluorophores and some of the most promising new fluorophores. Since their introduction in the late 1990's, these product designs have served as the starting point for custom designed filters for market leading instruments including DNA Sequencers, Cytometers, MicroPlate Readers, In vivo Imagers, and Thermocyclers. We are now making these OEM proven designs available to the individual researcher through our catalog offering of High Performance Filter sets for Epi-Fluorescence microscopy. HPF™ filters are manufactured using Newport's patented Stabilife® coating technology which provides excellent spectral performance, spectral stability, and physical durability.

HPF™ filter sets have been manufactured to provide maximum performance using several key design features including exceptionally high passband transmission, maximized out-of-band blocking including extended range blocking on excitation filters, extremely steep slopes from rejection to transmission regions, optical finishing for maximum image rendition including precision grinding and polishing and anti-reflection coatings, and filter bandpass specifications of center wavelength and bandwidth providing maximum image brightness and minimum background.

Filter Set Selection

Newport's HPF™ Fluorescence Filter Sets are listed in two tables. The Fluorophore Reference table lists the recommended filter set for each listed fluorophore. The Numerical Reference table identifies all of the fluorophores that can be utilized with each filter set. Spectral performance of each filter set is provided for reference in a series of data curves following the tables.

Custom Products and Services

Custom sizes of HPF™ filter sets as well as mounting services are available upon request. Please consult a sales engineer for details.

Trademark Information

Alexa Fluor®, BODIPY®, Calcium Crimson™, LysoSensor™,

MitoTracker®, Oregon Green®, Pacific Blue™, Rhodamine Green™,

and Texas Red® are trademarks of Molecular Probes, Inc.

Cy is a trademark of GE Healthcare, Ltd.

Stabilife®, XM™ and HPF™ are trademarks of Newport Corporation.